Document 0501
 DOCN  M9480501
 TI    Direct injection of a recombinant retroviral vector induces human
       immunodeficiency virus-specific immune responses in mice and nonhuman
       primates.
 DT    9410
 AU    Irwin MJ; Laube LS; Lee V; Austin M; Chada S; Anderson CG; Townsend K;
       Jolly DJ; Warner JF; Department of Immunobiology, Viagene, Inc., San
       Diego, California; 92121.
 SO    J Virol. 1994 Aug;68(8):5036-44. Unique Identifier : AIDSLINE
       MED/94309169
 AB    The cytotoxic T-lymphocyte (CTL) response plays an important role in
       controlling the severity and duration of viral infections. Immunization
       by direct in vivo administration of retroviral vector particles
       represents an efficient means of introducing and expressing genes and,
       subsequently, the proteins they encode in vivo in mammalian cells. In
       this manner foreign proteins can be provided to the endogenous, class I
       major histocompatibility complex antigen presentation pathway leading to
       CTL activation. A nonreplicating recombinant retroviral vector, encoding
       the human immunodeficiency virus type 1 (HIV-1) IIIB envelope and rev
       proteins, has been developed and examined for stimulation of immune
       responses in mouse, rhesus macaque, and baboon models. Animals were
       immunized by direct intramuscular injection of the retroviral vector
       particles. Vector-immunized mice, macaques, and baboons generated
       long-lived CD8+, major histocompatibility complex-restricted CTL
       responses that were HIV-1 protein specific. The CTL responses were found
       to be dependent on the ability of the retroviral vector to transduce
       cells. The vector also elicited HIV-1 envelope-specific antibody
       responses in mice and baboons. These studies demonstrate the ability of
       a retroviral vector encoding HIV-1 proteins to stimulate cellular and
       humoral immune responses and suggest that retrovector immunization may
       provide an effective means of inducing or augmenting CTL responses in
       HIV-1-infected individuals.
 DE    Animal  Female  Genetic Vectors/ADMINISTRATION & DOSAGE/*IMMUNOLOGY  HIV
       Antibodies/BIOSYNTHESIS/IMMUNOLOGY  HIV-1/GENETICS/*IMMUNOLOGY  Macaca
       mulatta  Mice  Mice, Inbred BALB C  Papio  Support, Non-U.S. Gov't
       T-Lymphocytes, Cytotoxic/*IMMUNOLOGY  Vaccination  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).